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KMID : 0368419930360020177
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Journal of Plant Biology
1993 Volume.36 No. 2 p.177 ~ p.182
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Isolation of Symbiotic Frankia EuIK1 Strain from Root Nodule of Elaeagnus umbellata
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Abstract
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The root nodules of Elaeagnus umbellata were coralloid-shape due to repeated dichotomous branching of nodule meristem. The filamentous endophyte with vesicle cluster ranging from 30 ㎛ to 60 ㎛ in diameter was present only in the cortical cells. The isolated endophytes in vitro culture showed typical Frankia morphology, consisting of highly branched hyphae ranging from 0.8 ㎛ to 1.0 ㎛ in diameter, terminal and intraphyphal sporangia varing in shape and size up to 60 ㎛ in length and laminated vesicles. Its infectivity and effectivity were confirmed by induction of nitrogen-fixing root nodules on the inoculated seedlings of two Elaeagnus species. Consequently, the isolate was confirmed as a true symbiont of Elaeagnus umbellata root nodule and named Frankia EuIK1.
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